宿主细胞参与小型病毒复制。I. M-13在一株脱氧核糖核酸合成存在温度敏感损伤的大肠杆菌中的复制。
Host cell participation in small virus replication. I. Replication of M-13 in a strain of Escherichia coli with a temperature-sensitive lesion in deoxyribonucleic acid synthesis.
作者信息
Primrose S B, Brown L R, Dowell C E
出版信息
J Virol. 1968 Nov;2(11):1308-14. doi: 10.1128/JVI.2.11.1308-1314.1968.
Abstract
The replication of M-13 in a strain of Escherichia coli with a thermosensitive lesion in deoxyribonucleic acid synthesis was studied. M-13 failed to replicate at the restrictive temperature, even when the parental replicative form was allowed to form at the permissive temperature. When cells which were actively producing phage at the permissive temperature were shifted to the restrictive temperature, phage production continued. The incorporation of radioactive label into phage particles at 42 C indicated that continued single-strand synthesis was unaffected by the lesion in the host cell.
摘要
研究了M-13在脱氧核糖核酸合成中存在温度敏感损伤的大肠杆菌菌株中的复制情况。即使允许在允许温度下形成亲本复制型，M-13在限制温度下也无法复制。当在允许温度下活跃产生噬菌体的细胞转移到限制温度时，噬菌体的产生仍在继续。在42℃下将放射性标记掺入噬菌体颗粒表明，连续的单链合成不受宿主细胞损伤的影响。
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