评估摩根和弗里奥尼斯梅毒螺旋体计数方法的统计学方法。
Statistical approach to evaluating the method of Morgan and Vryonis for enumerating Treponema pallidum.
作者信息
Artley C W, Clark J W
出版信息
Appl Microbiol. 1969 May;17(5):665-70. doi: 10.1128/am.17.5.665-670.1969.
The contributions of four components to the error in the microscopic enumeration of spirochetes (Treponema pallidum) by the method of Morgan and Vryonis were determined. Where N(s) = the number of slides counted, N(r) = the number of recounts of 50 fields per slide, N(p) = the number of pipettes used, and N(e) = the total number of counts of 50 fields made, the coefficient of variation is 2 times the square root of (17.5(2)/N(s) + 18.6(2)/N(r) + 14.8(2)/N(p) + 6.1(2)/N(e)) with 95% confidence.
确定了通过摩根和弗里奥尼斯方法对螺旋体（梅毒螺旋体）进行显微镜计数时，四个组成部分对误差的贡献。其中，N(s)为计数的玻片数量，N(r)为每张玻片50个视野的重新计数次数，N(p)为使用的移液管数量，N(e)为50个视野的总计数次数，变异系数为17.5²/N(s) + 18.6²/N(r) + 14.8²/N(p) + 6.1²/N(e)之和的平方根的2倍，置信度为95%。
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