梅毒螺旋体抗体的自动化定量微量血凝试验。
Automated, quantitative microhemagglutination assay for Treponema pallidum antibodies.
作者信息
Cox P M, Logan L C, Norins L C
出版信息
Appl Microbiol. 1969 Sep;18(3):485-9. doi: 10.1128/am.18.3.485-489.1969.
Abstract
An automated, quantitative microhemagglutination assay for antibodies to Treponema pallidum was developed by using T. pallidum-sensitized erythrocytes and an automatic serial-dilution instrument. Reactivity was found in sera from 54 rabbits and 6 chimpanzees infected with T. pallidum. Reactivity was also found in sera from animals infected with T. pertenue, T. carateum, and T. cuniculi. No reactivity was found in sera from 75 normal rabbits or from 129 rabbits immunized with cultivatable treponemes or a variety of other bacteria. In approximately 3 min, 13 twofold serial dilutions of each of 8 preabsorbed sera and the addition of sensitized erythrocytes to each dilution were accomplished automatically. The automated assay can serve as a research tool in quantitating antibodies to pathogenic treponemes, and evaluation of its clinical usefulness seems warranted.
摘要
利用梅毒螺旋体致敏红细胞和自动连续稀释仪，开发了一种用于检测梅毒螺旋体抗体的自动化定量微量血凝试验。在感染梅毒螺旋体的54只兔子和6只黑猩猩的血清中发现了反应性。在感染雅司螺旋体、品他螺旋体和兔密螺旋体的动物血清中也发现了反应性。在75只正常兔子或用可培养的密螺旋体或多种其他细菌免疫的129只兔子的血清中未发现反应性。在大约3分钟内，自动完成了8份预先吸收血清中每份血清的13次两倍连续稀释，并向每次稀释液中加入致敏红细胞。该自动化试验可作为定量检测致病性密螺旋体抗体的研究工具，对其临床实用性进行评估似乎是有必要的。
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