转运RNA种类的化学修饰。用雷尼镍进行脱硫
Chemical modifications of transfer RNA species. Desulfurization with Raney nickel.
作者信息
Hecht S M, Kirkegaard L H, Bock R M
出版信息
Proc Natl Acad Sci U S A. 1971 Jan;68(1):48-51. doi: 10.1073/pnas.68.1.48.
Abstract
A single, modified nucleoside in Escherichia coli tRNA, 6-(3-methyl-2-butenylamino)-2-methyl-thio-9-beta-D-ribofuranosylpurine, has been desulfurized with Raney nickel to afford its probable biosynthetic precursor. The limitations of the reaction at the nucleoside and tRNA levels and its lack of inhibition of the amino acid acceptor activity of tRNA are described.
摘要
大肠杆菌转运核糖核酸（tRNA）中的一种单一修饰核苷，即6-(3-甲基-2-丁烯基氨基)-2-甲基硫代-9-β-D-呋喃核糖基嘌呤，已用雷尼镍进行脱硫反应，以得到其可能的生物合成前体。文中描述了该反应在核苷和tRNA水平上的局限性，以及它对tRNA氨基酸接受活性缺乏抑制作用的情况。
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