在碘化钾梯度上纯化的作为RNA-DNA杂交体的特定痘苗病毒RNA的翻译。
Translation of specific vaccinia virus RNAs purified as RNA-DNA hybrids on potassium iodide gradients.
作者信息
Cooper J A, Moss B
出版信息
Nucleic Acids Res. 1979 Aug 10;6(11):3599-612. doi: 10.1093/nar/6.11.3599.
A procedure has been developed for purifying specific mRNAs by hybridization to fragments of DNA and isolation of the hybrids by potassium iodide equilibrium buoyant density centrifugation. The hybrids obtained are essentially free of unhybridized RNA as well as double-stranded RNA. Moreover, the RNA in the hybrids is undamaged and can be translated in vitro. Application of this procedure to mapping vaccinia virus genes is described. A total of 34 polypeptides have been assigned to three regions of the viral genome.
已开发出一种程序，通过与DNA片段杂交来纯化特定的mRNA，并通过碘化钾平衡浮力密度离心法分离杂交体。所获得的杂交体基本上不含未杂交的RNA以及双链RNA。此外，杂交体中的RNA未受损，并且可以在体外进行翻译。本文描述了该程序在痘苗病毒基因图谱绘制中的应用。总共34种多肽已被定位到病毒基因组的三个区域。
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