肉毒梭菌213B菌株中细菌素博蒂辛B编码基因的克隆、核苷酸序列及表达
Cloning, nucleotide sequence, and expression of the gene encoding the bacteriocin boticin B from Clostridium botulinum strain 213B.
作者信息
Dineen S S, Bradshaw M, Johnson E A
机构信息
Department of Food Microbiology and Toxicology, Food Research Institute, University of Wisconsin, Madison, Wisconsin 53706, USA.
出版信息
Appl Environ Microbiol. 2000 Dec;66(12):5480-3. doi: 10.1128/AEM.66.12.5480-5483.2000.
Abstract
Boticin B is a heat-stable bacteriocin produced by Clostridium botulinum strain 213B that has inhibitory activity against various strains of C. botulinum and related clostridia. The gene encoding the bacteriocin was localized to a 3.0-kb HindIII fragment of an 18. 8-kb plasmid, cloned, and sequenced. DNA sequencing revealed the boticin B structural gene, btcB, to be an open reading frame encoding 50 amino acids. A C. botulinum strain 62A transconjugant containing the HindIII fragment inserted into a clostridial shuttle vector expressed boticin B, although at much lower levels than those observed in C. botulinum 213B. To our knowledge, this is the first demonstration and characterization of a bacteriocin from toxigenic group I C. botulinum.
摘要
肉毒菌素B是由肉毒梭菌213B菌株产生的一种热稳定细菌素，对多种肉毒梭菌菌株及相关梭菌具有抑制活性。编码该细菌素的基因定位于一个18.8 kb质粒的3.0 kb HindIII片段上，进行了克隆和测序。DNA测序显示，肉毒菌素B结构基因btcB是一个编码50个氨基酸的开放阅读框。含有插入到梭菌穿梭载体中的HindIII片段的肉毒梭菌62A转接合子表达了肉毒菌素B，尽管表达水平远低于在肉毒梭菌213B中观察到的水平。据我们所知，这是首次对产毒I群肉毒梭菌的细菌素进行证明和表征。
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