用鞭毛血清对沙门氏菌进行免疫荧光染色。
Immunofluorescent staining of Salmonella species with flagellar sera.
作者信息
Goepfert J M, Hicks R
出版信息
Appl Microbiol. 1969 Oct;18(4):612-7. doi: 10.1128/am.18.4.612-617.1969.
Abstract
Salmonellae stained with flagellar sera by either the direct or indirect fluorescent-antibody technique fluoresced at the cell surface. Investigations showed that fimbriae, flagellar material, and capsule antigens were not the participating antigens in this reaction. Cell surface staining was inhibited by mannose but was unaffected by glucose, galactose, lactose, maltose, and sucrose. Specific absorption of the flagellar sera by intact cells and purified somatic antigen showed that O antigens were the site of the surface staining. The advantages of using flagellar sera in fluorescent-antibody screening of materials for the presence of salmonellae are discussed.
摘要
通过直接或间接荧光抗体技术用鞭毛血清染色的沙门氏菌在细胞表面发出荧光。研究表明，菌毛、鞭毛物质和荚膜抗原不是该反应中的参与抗原。细胞表面染色被甘露糖抑制，但不受葡萄糖、半乳糖、乳糖、麦芽糖和蔗糖的影响。完整细胞和纯化的菌体抗原对鞭毛血清的特异性吸收表明，O抗原是表面染色的部位。文中讨论了在荧光抗体筛选材料中是否存在沙门氏菌时使用鞭毛血清的优点。
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