Schwab J H
J Bacteriol. 1965 Nov;90(5):1412-9. doi: 10.1128/jb.90.5.1412-1419.1965.
Schwab, John H. (University of North Carolina, Chapel Hill). Biological properties of streptococcal cell-wall particles. II. Purification by density gradient column electrophoresis. J. Bacteriol. 90:1412-1419. 1965.-Sucrose gradient column electrophoresis was utilized as a preparative method to purify streptococcal cell-wall fragments. Enzyme-treated cell-wall preparations could be resolved into two major fractions. All of the cell-wall material was in the fraction of slower electrophoretic mobility. The latent time between injection and appearance of nodular lesions in rabbit skin was significantly shorter with this fraction, compared with crude material. In addition, the incidence of relapses of the nodular lesion was reduced with this purified preparation. Addition of the faster electrophoretic fraction back to the slower cell wall-containing fraction prolonged the latent time between injection and appearance of nodules, and increased the incidence of relapses. The latent time was directly proportional to the ratio of the two fractions. Antigens of the electrophoretic fast fraction were distinct from the C polysaccharide and mucopeptide antigens of the cell wall.
施瓦布，约翰·H.（北卡罗来纳大学教堂山分校）。链球菌细胞壁颗粒的生物学特性。II. 通过密度梯度柱电泳进行纯化。《细菌学杂志》90:1412 - 1419。1965年。——蔗糖梯度柱电泳被用作一种制备方法来纯化链球菌细胞壁片段。经酶处理的细胞壁制剂可被分离成两个主要部分。所有细胞壁物质都在电泳迁移较慢的部分。与粗制品相比，该部分在兔皮中注射至出现结节性病变的潜伏期明显更短。此外，使用这种纯化制剂可降低结节性病变复发的发生率。将电泳迁移较快的部分添加回含细胞壁较慢的部分会延长注射至出现结节的潜伏期，并增加复发率。潜伏期与这两个部分的比例成正比。电泳快速部分的抗原与细胞壁的C多糖和粘肽抗原不同。
