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超速离心机垂直转子在蔗糖密度梯度上检测风疹特异性IgM中的应用。

Use of the ultracentrifuge vertical rotor in the detection of rubella-specific IgM on a sucrose density gradient.

作者信息

Luton P, Ridgway G L

出版信息

J Clin Pathol. 1979 Sep;32(9):931-4. doi: 10.1136/jcp.32.9.931.

DOI:10.1136/jcp.32.9.931
PMID:512054
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1145854/
Abstract

A comparison was made of the performance of swing-out and vertical ultracentrifuge rotors in the detection of rubella-specific IgM on a sucrose density gradient. Tests were performed on 30 sera, of which 11 were found to contain rubella-specific IgM by both methods. The centrifugation time for the swing-out rotor was 16 hours at 35,000 rpm. This was reduced to 2 hours using the vertical rotor at 50,000 rpm. Routine use of the vertical rotor would allow a reduction in the time taken to perform the test, increase the number of sera tested each time, and reduce wear on the ultracentrifuge.

摘要

对用于在蔗糖密度梯度上检测风疹特异性IgM的甩平式和垂直式超速离心机转子的性能进行了比较。对30份血清进行了检测,其中11份血清通过两种方法均检测到风疹特异性IgM。甩平式转子在35000转/分钟的转速下离心时间为16小时。使用垂直式转子在50000转/分钟的转速下,离心时间缩短至2小时。常规使用垂直式转子将减少检测所需时间,每次增加检测的血清数量,并减少超速离心机的磨损。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b4b/1145854/c278cc7bae2d/jclinpath00457-0075-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b4b/1145854/74bea882b9aa/jclinpath00457-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b4b/1145854/f3af3acf01eb/jclinpath00457-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b4b/1145854/c278cc7bae2d/jclinpath00457-0075-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b4b/1145854/74bea882b9aa/jclinpath00457-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b4b/1145854/f3af3acf01eb/jclinpath00457-0075-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b4b/1145854/c278cc7bae2d/jclinpath00457-0075-b.jpg

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J Clin Pathol. 1979 Sep;32(9):931-4. doi: 10.1136/jcp.32.9.931.
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引用本文的文献

1
Demonstration of rubella-specific IgM by serum ultracentrifugation on sucrose gradients: comparison of a vertical and a swinging bucket rotor.通过在蔗糖梯度上进行血清超速离心法检测风疹特异性 IgM:垂直转子与摆动式转头的比较
Med Microbiol Immunol. 1982;171(2):69-75. doi: 10.1007/BF02124913.

本文引用的文献

1
A simplified method for the detection of rubella-specific IgM employing sucrose density fractionation and 2-mercaptoethanol.一种采用蔗糖密度分级分离和2-巯基乙醇检测风疹特异性IgM的简化方法。
J Hyg (Lond). 1974 Dec;73(3):329-40. doi: 10.1017/s0022172400042674.
2
Rubella: a method for rapid diagnosis of a recent infection by demonstration of the IgM antibodies.风疹:一种通过检测IgM抗体来快速诊断近期感染的方法。
Br Med J. 1968 Jan 27;1(5586):221-3. doi: 10.1136/bmj.1.5586.221.
3
The detection of specific IgM antibodies following infection with rubella virus.
风疹病毒感染后特异性IgM抗体的检测。
J Clin Pathol. 1975 May;28(5):377-82. doi: 10.1136/jcp.28.5.377.
4
Evaluation of a simplified sucrose gradient method for the detection of rubella-specific IgM in routine diagnostic practice.一种简化蔗糖梯度法在常规诊断实践中检测风疹特异性 IgM 的评估。
J Med Virol. 1978;2(2):153-63. doi: 10.1002/jmv.1890020210.