Bottone E J, Sandhu K K, Pisano M A
J Clin Microbiol. 1979 Oct;10(4):433-6. doi: 10.1128/jcm.10.4.433-436.1979.
Yersinia enterocolitica and related Yersinia species share many temperature-dependent biological attributes. The present report documents temperature-dependent production of a bacteriocin-like substance only among Yersinia strains which ferment L-rhamnose, raffinose, and melibiose and which have been tentatively designed Y. intermedia. When tested by the "lawn-spotting" technique at 25 and 37 degrees C, 7 of 15 Y. intermedia strains produced a bacteriocin only at 25 degrees C with a uniform spectrum of activity against 2 strains of Y. enterocolitica, 1 rhamnose-positive, raffinose-negative, melibiose-negative strain tentatively designated Y. frederiksenii, 8 Y. intermedia, and 3 sucrose- and acetylmethylcarbinol-negative yersinial isolates tested. Bacteriocin-like activity was not detected among the Y. enterocolitica, Y. frederiksenii, or sucrose-negative yersinial strains tested. The exclusive activity of the Y. intermedia antibacterial substance only against yersiniae and not against other representative Enterobacteriaceae tested supports the placement of these microorganisms within the genus Yersinia and further establishes the singularity of Y. intermedia.
小肠结肠炎耶尔森菌及相关耶尔森菌属菌种具有许多温度依赖性生物学特性。本报告记录了仅在发酵L - 鼠李糖、棉子糖和蜜二糖且暂定为中间型耶尔森菌的耶尔森菌菌株中存在温度依赖性的类细菌素物质产生情况。当采用“菌苔点滴”技术在25℃和37℃进行检测时，15株中间型耶尔森菌中有7株仅在25℃产生细菌素，其对2株小肠结肠炎耶尔森菌、1株暂定为弗氏耶尔森菌的鼠李糖阳性、棉子糖阴性、蜜二糖阴性菌株、8株中间型耶尔森菌以及3株蔗糖和乙酰甲基甲醇阴性的耶尔森菌分离株具有一致的活性谱。在所检测的小肠结肠炎耶尔森菌、弗氏耶尔森菌或蔗糖阴性的耶尔森菌菌株中未检测到类细菌素活性。中间型耶尔森菌抗菌物质仅对耶尔森菌有活性而对所检测的其他代表性肠杆菌科细菌无活性，这支持将这些微生物归为耶尔森菌属，并进一步确立了中间型耶尔森菌的独特性。
