Ovcinnikov N M
Bull World Health Organ. 1965;33(2):197-208.
The standard TPI test is a complicated technique that can be performed only by highly qualified staff in well-equipped laboratories. This has greatly restricted its application. The author describes a simplified method that does not require complicated apparatus or the use of a gas mixture. The test is performed in diluting pipettes of the type used in making white blood cell counts. The serum to be tested and treponemal suspension containing active complement are taken up successively into the pipette, which is then closed with a rubber ring, shaken and placed in the incubator. A parallel series of control pipettes with inactivated complement is also set up. After incubation, counts of motile and non-motile treponemes are made in the usual way. Comparative studies have shown that the results obtained with this simplified test are comparable in accuracy to those of the standard method. The medium used by the author for preparing the treponemal suspension is also very simple in composition. The technique can therefore be performed in any laboratory that has qualified staff and facilities for keeping rabbits.
标准的梅毒螺旋体活动抑制（TPI）试验是一项复杂的技术，只有设备精良的实验室中训练有素的人员才能进行。这极大地限制了它的应用。作者描述了一种简化方法，该方法不需要复杂的仪器，也无需使用混合气体。该试验在用于进行白细胞计数的那种稀释移液管中进行。将待测血清和含有活性补体的梅毒螺旋体悬液依次吸入移液管，然后用橡胶圈封闭，摇晃后放入培养箱。同时还设置了一系列补体已灭活的平行对照移液管。培养后，按常规方法对活动和不活动的梅毒螺旋体进行计数。对比研究表明，这种简化试验所获得的结果在准确性上与标准方法相当。作者用于制备梅毒螺旋体悬液的培养基成分也非常简单。因此，任何有合格人员和饲养兔子设施的实验室都可以进行这项技术操作。
