人类补体的第八成分（C8）：分离、特性鉴定及溶血效率
The eighth component of human complement (C8): isolation, characterization, and hemolytic efficiency.
作者信息
Manni J A, Müller-Eberhard H J
出版信息
J Exp Med. 1969 Nov 1;130(5):1145-60. doi: 10.1084/jem.130.5.1145.
Abstract
A method was described allowing the isolation of the eighth component of complement, C8, from human serum. C8 is an immunochemically definable, heretofore unrecognized serum protein with a molecular weight of approximately 150,000 and an electrophoretic mobility of a gamma(1)-globulin. It may be obtained with 5% yield as a 5000-fold purified, physicochemically and immunochemically homogeneous protein. Under optimal experimental conditions, a few molecules of C8 were found sufficient for lysis of a cell.
摘要
本文描述了一种从人血清中分离补体第八成分C8的方法。C8是一种免疫化学可定义的、此前未被识别的血清蛋白，分子量约为150,000，电泳迁移率为γ(1)-球蛋白。它可以以5%的产率获得，是一种经过5000倍纯化的、物理化学和免疫化学均一的蛋白质。在最佳实验条件下，发现少量C8分子就足以裂解细胞。
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本文引用的文献
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ISOLATION OF BETA IF-GLOBULIN FROM HUMAN SERUM AND ITS CHARACTERIZATION AS THE FIFTH COMPONENT OF COMPLEMENT.从人血清中分离β-免疫球蛋白并将其鉴定为补体的第五成分。
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Isolation of a thermolabile serum protein which precipitates gamma-globulin aggregates and participates in immune hemolysis.一种热不稳定血清蛋白的分离，该蛋白可沉淀γ-球蛋白聚集体并参与免疫溶血。
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