Phillips J H
J Bacteriol. 1969 Nov;100(2):695-700. doi: 10.1128/jb.100.2.695-700.1969.
Methyl-deficient transfer ribonucleic acid (tRNA) is found in certain methionine auxotrophs of Saccharomyces cerevisiae during logarithmic growth (at one generation time before the late growth phase) and during residual growth in the absence of exogenous methionine. The former effect seems to be accounted for by the general increase in RNA synthesis that occurs at the time; there is no specific synthesis of tRNA in the absence of ribosomal RNA synthesis, nor is the methyl group deficiency limited to a single tRNA species. During methionine starvation, all species of tRNA are methyl-deficient, but this occurs only in strains with certain blocks in the methionine pathway. The kinetics of disappearance of the methyl group donor, S-adenosylmethionine, during starvation of D73 (which accumulates methyl-deficient tRNA), do not differ from other strains, but D73 loses the methylase inhibitor, S-adenosylhomocysteine, much more slowly.
甲基缺乏的转移核糖核酸（tRNA）在酿酒酵母的某些甲硫氨酸营养缺陷型菌株的对数生长期（在生长后期前一代时间）以及在无外源甲硫氨酸的残留生长期间被发现。前一种效应似乎是由当时RNA合成的普遍增加所导致；在没有核糖体RNA合成的情况下，不存在tRNA的特异性合成，甲基缺乏也不限于单一的tRNA种类。在甲硫氨酸饥饿期间，所有种类的tRNA都是甲基缺乏的，但这仅发生在甲硫氨酸途径有某些阻断的菌株中。在D73（积累甲基缺乏的tRNA）饥饿期间，甲基供体S-腺苷甲硫氨酸消失的动力学与其他菌株没有差异，但D73失去甲基化酶抑制剂S-腺苷高半胱氨酸的速度要慢得多。
