Tippett L O, Zeleznick L D, Robb C A
Appl Microbiol. 1970 Sep;20(3):342-5. doi: 10.1128/am.20.3.342-345.1970.
A pH indicator and dextrose were incorporated into growth media as a modification of microbial microtiter methods for determining the minimal inhibitory concentration of antimicrobial drugs. This modified method was tested to evaluate the ease of reading end points by changes in the indicator color. Application of the procedure to two media, three indicators, and eight species of bacteria indicated that definitive end points could be reached as a result of indicator color change caused by acid production during bacterial growth. This method is accurate and reproducible. It is a modification which eliminates a need for plating and facilitates the reading of minimal inhibitory concentration end points.
将pH指示剂和葡萄糖加入生长培养基中，作为微生物微量滴定法的一种改进，用于测定抗菌药物的最低抑菌浓度。对这种改进方法进行了测试，以评估通过指示剂颜色变化读取终点的难易程度。将该程序应用于两种培养基、三种指示剂和八种细菌，结果表明，由于细菌生长过程中产酸导致指示剂颜色变化，能够达到明确的终点。该方法准确且可重复。它是一种改进方法，无需进行平板接种，便于读取最低抑菌浓度终点。
