组蛋白H3和H4的八聚体与核小体大小的DNA形成紧密复合物。
An octamer of histones H3 and H4 forms a compact complex with DNA of nucleosome size.
作者信息
Simon R H, Camerini-Otero R D, Felsenfeld G
出版信息
Nucleic Acids Res. 1978 Dec;5(12):4805-18. doi: 10.1093/nar/5.12.4805.
Abstract
Equimolar mixtures of histones H3 and H4 have been reconstituted onto DNA of nucleosome core size. Two distinct complexes are formed in a relative abundance that depends on the starting ratio of H3 + H4 to DNA. One of these complexes contains two H3-H4 dimers for each DNA molecule, and has a sedimentation coefficient of 7.5S. The other complex contains an octamer consisting of four H3-H4 dimers, and has a sedimentation coefficient of 10.4S. On the basis of these measurements, we conclude that the octamer complex (but not the tetramer complex) is a fully folded, compact structure resembling the nucleosome.
摘要
组蛋白H3和H4的等摩尔混合物已被重组到核小体核心大小的DNA上。形成了两种不同的复合物，其相对丰度取决于H3 + H4与DNA的起始比例。其中一种复合物每个DNA分子包含两个H3-H4二聚体，沉降系数为7.5S。另一种复合物包含由四个H3-H4二聚体组成的八聚体，沉降系数为10.4S。基于这些测量结果，我们得出结论，八聚体复合物（而非四聚体复合物）是一种类似于核小体的完全折叠的紧密结构。
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