Lúcke H, Berner W, Menge H, Murer H
Pflugers Arch. 1978 Mar 20;373(3):243-8. doi: 10.1007/BF00580831.
Uptake of D- and L-glucose, and fructose by purified brush border membrane vesicles isolated from human small intestine was studied using a rapid filtration technique. The uptake of D-glucose by the vesicles was osmotically sensitive and represented transport into an intravesicular space and not binding to the membranes. Transport of both, D- and L-glucose was inhibited by phlorizin. Uptake of D-fructose into the brush border vesicles was not stimulated by sodium. In the presence of a sodium gradient D-glucose was taken up 5 times faster than L-glucose. The amount of D-glucose transported into the vesicles in the presence of a sodium gradient was transiently higher than the amount of D-glucose taken up at equilibrium (over-shoot). D-Glucose transport was stimulated only by a sodium gradient; other monovalent cations had no effect. In the presence of a sodium gradient D-glucose transport was increased by the nearly impermeable anion sulfate as compared with uptake of D-glucose in the presence of a sodium chloride gradient. This indicates an influence of the electrical membrane potential on the sodium coupled non-electrolyte transport.
采用快速过滤技术研究了从人小肠分离的纯化刷状缘膜囊泡对D-葡萄糖、L-葡萄糖和果糖的摄取。囊泡对D-葡萄糖的摄取对渗透压敏感，代表其转运至囊泡内空间而非与膜结合。根皮苷可抑制D-葡萄糖和L-葡萄糖的转运。刷状缘囊泡对D-果糖的摄取不受钠的刺激。在存在钠梯度的情况下，D-葡萄糖的摄取速度比L-葡萄糖快5倍。在存在钠梯度的情况下，转运至囊泡内的D-葡萄糖量短暂高于平衡时摄取的D-葡萄糖量（过冲）。D-葡萄糖转运仅受钠梯度刺激；其他单价阳离子无作用。与存在氯化钠梯度时D-葡萄糖的摄取相比，在存在钠梯度的情况下，几乎不可渗透的阴离子硫酸盐可增加D-葡萄糖的转运。这表明膜电位对钠耦联非电解质转运有影响。
