Diacumakos E G, Garnjobst L, Tatum E L
J Cell Biol. 1965 Aug;26(2):427-43. doi: 10.1083/jcb.26.2.427.
Nuclear fractions isolated from mutants of Neurospora produced no effect when microinjected into mutants with complementary biochemical requirements. DNA isolated from the nuclear fractions similarly injected also had no effect. Mitochondrial fractions isolated from an abnormal inositolless strain (abn-1) produced drastic changes in the rate of growth, morphology, reproductive characteristics, and cytochrome spectra of normal inositolless strains when single hyphal compartments were microinjected and isolated, whereas the mitochondrial fractions of the wild type produced no effect. These results provide evidence for the transmission of biochemical and biological characters when mitochondria are transferred to new nucleocytoplasmic environments.
从粗糙脉孢菌突变体中分离出的细胞核组分,显微注射到具有互补生化需求的突变体中时没有产生影响。同样注射的从细胞核组分中分离出的DNA也没有影响。当单个菌丝间隔被显微注射并分离出来时,从异常的无肌醇菌株(abn-1)中分离出的线粒体组分,使正常无肌醇菌株的生长速率、形态、繁殖特性和细胞色素光谱发生了剧烈变化,而野生型的线粒体组分则没有影响。这些结果为线粒体转移到新的核质环境时生化和生物学特性的传递提供了证据。