Czlonkowska A
Eur J Clin Pharmacol. 1977 Dec 2;12(4):265-71. doi: 10.1007/BF00607425.
Humoral and cell-mediated immunity were studied in a group of patients with Wilson's disease not previously treated with D-penicillamine, and in a group of patients treated with the drug for more than two years. The previously untreated patients showed an exaggerated humoral immune response, i. e. increased levels of IgG and, IgM, higher titer of antibodies to Kunin's antigen, and depression of cell-mediated immunity, namely a decreased response to DNCB, decreased lymphocyte transformation after stimulation with Con A, PPD, Candida and streptokinase and a reduced response to streptokinase in the MIF test. After treatment the humoral response returned to normal, and in the case of IgA and antibodies to S. typhi O antigen, it even dropped below normal values. The cell-mediated immune response returned to normal with the exception of lymphocyte transformation by PHA and Candida albicans. In in vitro studies it was found that D-penicillamine had no influence on lymphocyte transformation when PHA and Con A were used as mitogens. With PPD as antigen, lymphocyte stimulation and migration inhibition were inhibited by concentrations of penicillamine ranging from 6 to 1000 microgram/ml.
对一组未曾接受过D-青霉胺治疗的威尔逊病患者以及一组接受该药治疗超过两年的患者的体液免疫和细胞介导免疫进行了研究。未经治疗的患者表现出过度的体液免疫反应，即IgG和IgM水平升高、对库宁抗原的抗体滴度更高，以及细胞介导免疫受到抑制，即对二硝基氯苯（DNCB）的反应降低、在刀豆蛋白A（Con A）、结核菌素纯蛋白衍生物（PPD）、白色念珠菌和链激酶刺激后淋巴细胞转化率降低，以及在巨噬细胞移动抑制因子（MIF）试验中对链激酶的反应减弱。治疗后，体液反应恢复正常，就IgA和抗伤寒杆菌O抗原的抗体而言，甚至降至正常值以下。细胞介导的免疫反应除了对PHA和白色念珠菌的淋巴细胞转化外，均恢复正常。在体外研究中发现，当使用PHA和Con A作为有丝分裂原时，D-青霉胺对淋巴细胞转化没有影响。以PPD作为抗原时，浓度为6至1000微克/毫升的青霉胺可抑制淋巴细胞刺激和迁移抑制。
