寄生曲霉无细胞提取物将柄曲霉素酶促转化为黄曲霉毒素B1 。
Enzymatic conversion of sterigmatocystin into aflatoxin B1 by cell-free extracts of Aspergillus parasiticus.
作者信息
Singh R, Hsieh D P
出版信息
Appl Environ Microbiol. 1976 May;31(5):743-5. doi: 10.1128/aem.31.5.743-745.1976.
Abstract
A cell-free extract, prepared from Aspergillus parasiticus ATCC 15517 grown in synthetic medium, was active in converting [14C]sterigmatocystin into aflatoxin B1 in the presence of reduced nicotinamide adenine dinucleotide phosphate. The activity was demonstrated by the time course of conversion and the linear dependence of the yield of product on enzyme concentrations. Optimum activity was obtained at pH 7.5 to 7.8 at 27 C. The results confirm sterigmatocystin as a biogenetic precursor of aflatoxin B1. Techniques were developed for enzymatic studies on aflatoxin biosynthesis.
摘要
从在合成培养基中生长的寄生曲霉ATCC 15517制备的无细胞提取物，在存在还原型烟酰胺腺嘌呤二核苷酸磷酸的情况下，能够将[14C]柄曲霉素转化为黄曲霉毒素B1。通过转化的时间进程以及产物产量对酶浓度的线性依赖性证明了该活性。在27℃、pH 7.5至7.8条件下可获得最佳活性。结果证实柄曲霉素是黄曲霉毒素B1的生物合成前体。开发了用于黄曲霉毒素生物合成酶学研究的技术。
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