El-Merzabani M M, Anwer-El-Aaser A, Zakhary N I
J Clin Chem Clin Biochem. 1977 Dec;15(12):715-8. doi: 10.1515/cclm.1977.15.1-12.715.
A simple method was developed for the determination of inorganic phosphorus in serum without deproteinization. The method is based on the use of formic acid as protein solubilizer and glycerol as stabilizer for the assay system. The optimal conditions for colour development were determined. The results obtained with the new method correlate well with those obtained after deproteinization of serum with trichloroacetic acid. The present method could be fully mechanized, and its application to the determination of serum phosphatases is discussed.
开发了一种无需去蛋白即可测定血清中无机磷的简单方法。该方法基于使用甲酸作为蛋白质增溶剂，甘油作为测定系统的稳定剂。确定了显色的最佳条件。用新方法获得的结果与用三氯乙酸对血清进行去蛋白后获得的结果相关性良好。本方法可完全机械化，并讨论了其在血清磷酸酶测定中的应用。
