对属于V NP基因家族的免疫球蛋白重链V区基因的分析。
Analysis of immunoglobulin heavy chain V-region genes belonging to the V NP-gene family.
作者信息
Blankenstein T, Zoebelein G, Krawinkel U
出版信息
Nucleic Acids Res. 1984 Sep 11;12(17):6887-900. doi: 10.1093/nar/12.17.6887.
Abstract
A method was devised to clone immunoglobulin VH-region genes located on selected restriction fragments from genomic DNA directly into M13 vectors for subsequent nucleotide sequence analysis. Ten recombinant M13 clones representing four so far unknown VH-region genes of the VNP-gene family have been analysed. Sequence comparison shows that these genes are closely related to other VH-genes of the VNP gene family. One of the VH-genes exhibits a so far unobserved unusual length of 100 2/3 codons and appears to be functional. Analysis of the variation of the isolated VH-genes suggests that framework and complementarity determining regions are exposed to separate types of selective pressures.
摘要
设计了一种方法，可将基因组DNA选定限制片段上的免疫球蛋白VH区基因直接克隆到M13载体中，以便进行后续核苷酸序列分析。已对代表VNP基因家族4个迄今未知VH区基因的10个重组M13克隆进行了分析。序列比较表明，这些基因与VNP基因家族的其他VH基因密切相关。其中一个VH基因呈现出迄今未观察到的100又2/3个密码子的异常长度，且似乎具有功能。对分离出的VH基因变异的分析表明，构架区和互补决定区受到不同类型的选择压力。
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