Buhk H J, Behrens B, Tailor R, Wilke K, Prada J J, Günthert U, Noyer-Weidner M, Jentsch S, Trautner T A
Gene. 1984 Jul-Aug;29(1-2):51-61. doi: 10.1016/0378-1119(84)90165-3.
Bacillus subtilis phage SPR codes for a DNA methyltransferase (Mtase) which methylates the 5' cytosine in the sequence GGCC and both cytosines in the sequence CCGG. A 2126-bp fragment of SPR DNA containing the Mtase gene has been sequenced. This fragment has only one significant open reading frame of 1347 bp, which corresponds to the Mtase gene. Within the sequence the Mtase promoter has been defined by S1 mapping. The size of the SPR Mtase predicted from the deduced amino acid composition is 49.9 kDal. This is in agreement with both the Mr of the purified enzyme and with that of the SPR Mtase gene product identified here by minicell technique. Base changes leading to mutants affected in Mtase activity were localized within the Mtase gene.
枯草芽孢杆菌噬菌体SPR编码一种DNA甲基转移酶（Mtase），该酶可使序列GGCC中的5'胞嘧啶以及序列CCGG中的两个胞嘧啶甲基化。已对包含Mtase基因的SPR DNA的2126 bp片段进行了测序。该片段只有一个1347 bp的重要开放阅读框，与Mtase基因相对应。在该序列中，Mtase启动子已通过S1图谱确定。根据推导的氨基酸组成预测的SPR Mtase大小为49.9 kDa。这与纯化酶的相对分子质量以及通过小细胞技术在此鉴定的SPR Mtase基因产物的相对分子质量均一致。导致Mtase活性受影响的突变体的碱基变化位于Mtase基因内。
