Shimotohno K, Wachsman W, Takahashi Y, Golde D W, Miwa M, Sugimura T, Chen I S
Proc Natl Acad Sci U S A. 1984 Nov;81(21):6657-61. doi: 10.1073/pnas.81.21.6657.
The nucleic acid sequence of the 3' region of human T-cell leukemia virus type II (HTLV-II) proviral DNA was determined using a HTLV-II proviral clone that could be recovered as infectious, transforming virus. The sequence data indicate a region of unknown function of approximately equal to 1.6 kilobase pairs in the 3' region, analogous to the X region previously identified in human T-cell leukemia virus type I (HTLV-I). Three overlapping open reading frames are present in the X region of HTLV-II. One of these open reading frames, Xc, is most likely to encode a protein product, because it has greater predicted amino acid sequence homology (78%) with the X-IV region of HTLV-I and a greater percentage of its base differences with X-IV at the third nucleotide position of codons than do the other open reading frames. Sequences of the X-region that include the open reading frames are conserved in two deletion mutants of HTLV-II, which are associated with a subline of Mo cells with a decreased dependence on fetal bovine serum.
利用一个可作为感染性转化病毒回收的人T细胞白血病病毒II型（HTLV-II）前病毒克隆，确定了HTLV-II前病毒DNA 3'区域的核酸序列。序列数据表明，3'区域存在一个功能未知的约1.6千碱基对的区域，类似于先前在人T细胞白血病病毒I型（HTLV-I）中鉴定出的X区域。HTLV-II的X区域存在三个重叠的开放阅读框。其中一个开放阅读框Xc最有可能编码一种蛋白质产物，因为与HTLV-I的X-IV区域相比，它具有更高的预测氨基酸序列同源性（78%），并且在密码子的第三个核苷酸位置与X-IV的碱基差异百分比比其他开放阅读框更高。包含开放阅读框的X区域序列在HTLV-II的两个缺失突变体中是保守的，这两个突变体与对胎牛血清依赖性降低的Mo细胞亚系相关。
