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人免疫球蛋白λ链可变序列的分子克隆

Molecular cloning of a human immunoglobulin lambda chain variable sequence.

作者信息

Tsujimoto Y, Croce C M

出版信息

Nucleic Acids Res. 1984 Nov 26;12(22):8407-14. doi: 10.1093/nar/12.22.8407.

DOI:10.1093/nar/12.22.8407
PMID:6095199
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320379/
Abstract

We have cloned a human V lambda cDNA sequence from an Ig lambda-producing human Burkitt lymphoma cell line (BL2) by taking advantage of a cloned constant region gene as a primer for cDNA synthesis instead of an oligo(dT) primer. The amino acid sequence deduced from the nucleotide sequence of V lambda clones is highly related to that of the NEW V lambda protein of subgroup I. Southern blot hybridization of human DNAs with the V lambda I probe showed at least 12 hybridizing V lambda fragments. These fragments are amplified in K562 cells which derive from a case of chronic myelogenous leukemia and contain an amplified c-abl oncogene and amplified C lambda sequences.

摘要

我们利用克隆的恒定区基因作为合成cDNA的引物而非寡聚（dT）引物，从产生Igλ的人伯基特淋巴瘤细胞系（BL2）中克隆了人Vλ cDNA序列。从Vλ克隆的核苷酸序列推导的氨基酸序列与I亚组的新型Vλ蛋白高度相关。用人DNA与VλI探针进行Southern印迹杂交显示至少有12个杂交的Vλ片段。这些片段在源自一例慢性粒细胞白血病且含有扩增的c-abl癌基因和扩增的Cλ序列的K562细胞中被扩增。

相似文献

1
Molecular cloning of a human immunoglobulin lambda chain variable sequence.人免疫球蛋白λ链可变序列的分子克隆
Nucleic Acids Res. 1984 Nov 26;12(22):8407-14. doi: 10.1093/nar/12.22.8407.
2
Cloning and sequencing of a rearranged V lambda gene from a Burkitt's lymphoma cell line expressing kappa light chains.从表达κ轻链的伯基特淋巴瘤细胞系中克隆和测序重排的Vλ基因。
Nucleic Acids Res. 1985 Jul 11;13(13):4921-34. doi: 10.1093/nar/13.13.4921.
3
Cloning and sequence analysis of an Ig lambda light chain mRNA expressed in the Burkitt's lymphoma cell line EB4.在伯基特淋巴瘤细胞系EB4中表达的Igλ轻链mRNA的克隆与序列分析。
Nucleic Acids Res. 1985 Apr 25;13(8):2931-41. doi: 10.1093/nar/13.8.2931.
4
V lambda and J lambda-C lambda gene segments of the human immunoglobulin lambda light chain locus are separated by 14 kb and rearrange by a deletion mechanism.人类免疫球蛋白λ轻链基因座的Vλ和Jλ-Cλ基因片段相隔14 kb，并通过缺失机制进行重排。
Eur J Immunol. 1991 Jun;21(6):1513-22. doi: 10.1002/eji.1830210627.
5
Chromosomal orientation of the lambda light chain locus: V lambda is proximal to C lambda in 22q11.λ轻链基因座的染色体定位：Vλ在22q11中靠近Cλ。
Nucleic Acids Res. 1985 Jan 25;13(2):381-7. doi: 10.1093/nar/13.2.381.
6
Restricted association of V and J-C gene segments for mouse lambda chains.小鼠λ链V和J-C基因片段的限制性组合
Proc Natl Acad Sci U S A. 1984 Apr;81(8):2484-8. doi: 10.1073/pnas.81.8.2484.
7
A complete immunoglobulin gene is created by somatic recombination.一个完整的免疫球蛋白基因是通过体细胞重组产生的。
Cell. 1978 Sep;15(1):1-14. doi: 10.1016/0092-8674(78)90078-8.
8
Amino acid and nucleotide sequences of variable regions of mouse immunoglobulin light chains of the lambda 3-subtype.λ3亚型小鼠免疫球蛋白轻链可变区的氨基酸和核苷酸序列。
J Immunol. 1984 Jul;133(1):471-5.
9
A third Ig light chain gene isotype in Xenopus laevis consists of six distinct VL families and is related to mammalian lambda genes.非洲爪蟾中的第三种免疫球蛋白轻链基因同种型由六个不同的VL家族组成，并且与哺乳动物的λ基因相关。
J Immunol. 1996 Aug 15;157(4):1544-50.
10
Organization of four mouse lambda light chain immunoglobulin genes.四个小鼠λ轻链免疫球蛋白基因的组织
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3765-9. doi: 10.1073/pnas.78.6.3765.

引用本文的文献

1
Burkitt's lymphoma is a malignancy of mature B cells expressing somatically mutated V region genes.伯基特淋巴瘤是一种表达体细胞突变V区基因的成熟B细胞恶性肿瘤。
Mol Med. 1995 Jul;1(5):495-505.
2
Physical location of the human immunoglobulin lambda-like genes, 14.1, 16.1, and 16.2.人类免疫球蛋白λ样基因的物理位置，14.1、16.1和16.2。
Immunogenetics. 1993;38(6):387-99. doi: 10.1007/BF00184519.
3
Haplotypes of the human immunoglobulin lambda IGLV7S1 and IGLV1S1 genes defined by restriction-site polymorphism and insertion/deletion of a 6-kb DNA fragment.

本文引用的文献

1
Transcriptional control of the expression of mouse globin genes in myeloma x erythroleukemia cell hybrids.骨髓瘤x红白血病细胞杂交体中小鼠珠蛋白基因表达的转录调控
Somatic Cell Genet. 1982 Mar;8(2):151-61. doi: 10.1007/BF01538674.
2
Localization of human immunoglobulin kappa light chain variable region genes to the short arm of chromosome 2 by in situ hybridization.通过原位杂交将人免疫球蛋白κ轻链可变区基因定位于2号染色体短臂。
Proc Natl Acad Sci U S A. 1982 Aug;79(16):4957-61. doi: 10.1073/pnas.79.16.4957.
3
DNA sequences of the joining regions of mouse lambda light chain immunoglobulin genes.
通过限制性酶切位点多态性和一个6 kb DNA片段的插入/缺失所定义的人类免疫球蛋白λ IGLV7S1和IGLV1S1基因的单倍型
Am J Hum Genet. 1993 Aug;53(2):518-25.
4
Mapping of the human lambda immunoglobulin variable gene subgroup 1.人类λ免疫球蛋白可变基因亚组1的定位
Immunogenetics. 1994;40(3):177-83. doi: 10.1007/BF00167077.
5
Characterization of a lymphoblastoid line deleted for lambda immunglobulin genes.缺失λ免疫球蛋白基因的淋巴母细胞系的特性分析
Immunogenetics. 1995;41(6):359-65. doi: 10.1007/BF00163993.
6
Cloning and sequencing of a rearranged V lambda gene from a Burkitt's lymphoma cell line expressing kappa light chains.从表达κ轻链的伯基特淋巴瘤细胞系中克隆和测序重排的Vλ基因。
Nucleic Acids Res. 1985 Jul 11;13(13):4921-34. doi: 10.1093/nar/13.13.4921.
7
Cloning and sequence analysis of an Ig lambda light chain mRNA expressed in the Burkitt's lymphoma cell line EB4.在伯基特淋巴瘤细胞系EB4中表达的Igλ轻链mRNA的克隆与序列分析。
Nucleic Acids Res. 1985 Apr 25;13(8):2931-41. doi: 10.1093/nar/13.8.2931.
8
Regional mapping of two human immunoglobulin V lambda genes and analysis of the V lambda locus in chronic myeloid leukaemia.两个人类免疫球蛋白Vλ基因的区域定位及慢性髓性白血病中Vλ基因座的分析
Nucleic Acids Res. 1985 Aug 26;13(16):5761-70. doi: 10.1093/nar/13.16.5761.
9
Human chromosome 22.人类22号染色体。
J Med Genet. 1987 Feb;24(2):65-78. doi: 10.1136/jmg.24.2.65.
10
Heterogeneity of chromosome 22 breakpoint in Philadelphia-positive (Ph+) acute lymphocytic leukemia.费城染色体阳性（Ph+）急性淋巴细胞白血病中22号染色体断点的异质性。
Proc Natl Acad Sci U S A. 1986 Mar;83(6):1807-11. doi: 10.1073/pnas.83.6.1807.
小鼠λ轻链免疫球蛋白基因连接区的DNA序列。
Proc Natl Acad Sci U S A. 1982 Jan;79(2):530-3. doi: 10.1073/pnas.79.2.530.
4
Chromosomal location of human kappa and lambda immunoglobulin light chain constant region genes.人类κ和λ免疫球蛋白轻链恒定区基因的染色体定位。
J Exp Med. 1982 May 1;155(5):1480-90. doi: 10.1084/jem.155.5.1480.
5
Assignment of the genes for human lambda immunoglobulin chains to chromosome 22.人类λ免疫球蛋白链基因定位于22号染色体。
Nature. 1981 Nov 12;294(5837):173-5. doi: 10.1038/294173a0.
6
Organization of four mouse lambda light chain immunoglobulin genes.四个小鼠λ轻链免疫球蛋白基因的组织
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3765-9. doi: 10.1073/pnas.78.6.3765.
7
Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments.克隆的人类和小鼠κ免疫球蛋白恒定区和J区基因在功能片段上保持同源性。
Cell. 1980 Nov;22(1 Pt 1):197-207. doi: 10.1016/0092-8674(80)90168-3.
8
Amplified C lambda and c-abl genes are on the same marker chromosome in K562 leukemia cells.在K562白血病细胞中，扩增的Cλ和c-abl基因位于同一条标记染色体上。
Proc Natl Acad Sci U S A. 1983 Dec;80(23):7289-92. doi: 10.1073/pnas.80.23.7289.
9
Transcriptional activation of an unrearranged and untranslocated c-myc oncogene by translocation of a C lambda locus in Burkitt.在伯基特淋巴瘤中，通过Cλ基因座的易位对未重排和未易位的c-myc癌基因进行转录激活。
Proc Natl Acad Sci U S A. 1983 Nov;80(22):6922-6. doi: 10.1073/pnas.80.22.6922.
10
Variation in V lambda genes in the genus Mus.小家鼠属中Vλ基因的变异
J Immunol. 1984 May;132(5):2638-43.