利用长期产生肿瘤病毒的细胞快速定量干扰素。
Rapid quantitation of interferon with chronically oncornavirus-producing cells.
作者信息
Aboud M, Weiss O, Salzberg S
出版信息
Infect Immun. 1976 Jun;13(6):1626-32. doi: 10.1128/iai.13.6.1626-1632.1976.
Abstract
The capacity of interferon to inhibit virus production in cells chronically infected with oncornavirus enabled us to develop a simple system for interferon quantitation that was independent of exogenous viral infection. The release of the virus to the culture medium was determined by its reverse transcriptase activity. The inhibitory effect of interferon in this system was linearly proportional to the log of its dilution over a range between 5 and 80% inhibiton. The sensitivity of the system was comparable to that of the vexicular stomatitis virus plaque reduction assay, whereas its reproducibility was found to be even better. This method is very rapid and can be completed within less than 24 h.
摘要
干扰素抑制细胞中被肿瘤病毒慢性感染后的病毒产生的能力，使我们能够开发出一种独立于外源性病毒感染的简单干扰素定量系统。通过逆转录酶活性来测定病毒释放到培养基中的情况。在该系统中，干扰素的抑制作用与其稀释倍数的对数在5%至80%抑制范围内呈线性比例关系。该系统的灵敏度与水泡性口炎病毒蚀斑减少试验相当，但其重现性更好。此方法非常快速，可在不到24小时内完成。
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