Lepper A W, Hart P D
Infect Immun. 1976 Aug;14(2):522-6. doi: 10.1128/iai.14.2.522-526.1976.
The peroxidase (PO) activity in nonelicited macrophages and in casein-elicited monocytes, obtained from peritoneal cavities of nonsensitized and BCG-sensitized mice and cultivated on glass for 1 or 2 h, was studied by light and electron microscopy, using the 3,3'-diaminobenzidine technique. These two types of glass-adherent peritoneal cells differed in PO activity. In macrophages, PO activity was predominantly in the nuclear envelope, rough endoplasmic reticulum, and occasionally in vesicles of the Golgi apparatus. In monocytes, PO activity was confined to cytoplasmic dense bodies resembling lysosomes, and was greater at 10 and 24 h after elicitation than at 96 h. The BCG sensitization did not significantly alter the proportion of cells with PO-positive granules in macrophages or monocytes from that observed in nonsensitized mice. From its lysosomal site, the PO in monocytes could come into contact with those microorganisms whose ingestion by these cells was followed by phagolysosome formation.
采用3,3'-二氨基联苯胺技术，通过光学显微镜和电子显微镜研究了从未致敏和卡介苗致敏小鼠腹腔中获取、并在玻璃上培养1或2小时的未激发巨噬细胞和酪蛋白激发单核细胞中的过氧化物酶（PO）活性。这两种贴壁于玻璃的腹腔细胞在PO活性方面存在差异。在巨噬细胞中，PO活性主要存在于核膜、粗面内质网，偶尔也存在于高尔基体的囊泡中。在单核细胞中，PO活性局限于类似溶酶体的细胞质致密体中，激发后10小时和24小时的活性高于96小时。卡介苗致敏并未显著改变巨噬细胞或单核细胞中具有PO阳性颗粒的细胞比例，与未致敏小鼠中观察到的比例相比无明显差异。单核细胞中的PO可从其溶酶体部位与那些被这些细胞吞噬后形成吞噬溶酶体的微生物接触。
