Pierce S K, Klinman N R, Maurer P H, Merryman C F
J Exp Med. 1980 Aug 1;152(2):336-49. doi: 10.1084/jem.152.2.336.
These studies were carried out to investigate the potential helper T cell repertoire specific for the random copolymer poly(L-Glu55,L-Ala35, L-Phe9)n(GL phi 9) of responder, nonresponder, and (responder x nonresponder)F1 murine strains. We tested the ability of these T cells to collaborate with dinitrophenyl (DNP)-specific primary and secondary B lymphocytes of each strain in response to the antigen CNP-GL phi 9 in the splenic-fragment culture system. The results of these experiments show that there are GL phi 9-specific T lymphocytes in the responder, nonresponder, and F1 strains; but that these three GL phi 9-specific T cell populations differ in their collaborative potential. Responder T cells are able to collaborate with their own syngeneic responder B cells as well as the allogeneic nonresponder B cells in a syngeneic fashion. The F1 T cell population resembles that of the nonresponder in its ability to collaborate with only responder B cells in a syngeneic fashion. Analysis carried out using appropriately selected mouse strains indicate that these results are unlikely to be a result of positive or negative allogeneic effects. The results obtained suggest that individuals within a given murine strain do possess the capacity to collaborate in a syngeneic fashion with B cells of any other MHC-allogeneic strain as well as their own MHC-identical B cells. The nonresponder status in the response to GL phi 9 appears to be the result of a deletion of T cells capable of recognizing antigen in the context of B cells of the nonresponder haplotype. Thus, the MHC gene products appear to play a determinative role in shaping the expressed helper T cell specificity repertoire within an individual mouse strain.
开展这些研究是为了调查应答、不应答以及（应答×不应答）F1小鼠品系针对随机共聚物聚（L-谷氨酸55、L-丙氨酸35、L-苯丙氨酸9）n（GL phi 9）的潜在辅助性T细胞库。我们在脾细胞片段培养系统中测试了这些T细胞与各品系的二硝基苯基（DNP）特异性初级和次级B淋巴细胞协同对抗抗原CNP-GL phi 9的能力。这些实验结果表明，应答、不应答和F1品系中存在GL phi 9特异性T淋巴细胞；但这三个GL phi 9特异性T细胞群体在协同潜力方面存在差异。应答性T细胞能够以同基因方式与自身同基因应答性B细胞以及异基因不应答性B细胞协同。F1 T细胞群体在以同基因方式仅与应答性B细胞协同的能力方面类似于不应答性T细胞群体。使用适当选择的小鼠品系进行的分析表明，这些结果不太可能是阳性或阴性异基因效应的结果。所获得的结果表明，给定小鼠品系内的个体确实具有以同基因方式与任何其他MHC异基因品系的B细胞以及自身MHC相同的B细胞协同的能力。对GL phi 9应答中的不应答状态似乎是能够在不应答单倍型的B细胞背景下识别抗原的T细胞缺失的结果。因此，MHC基因产物似乎在塑造单个小鼠品系内表达的辅助性T细胞特异性库方面发挥决定性作用。
