Kato K, Askenase P W
J Immunol. 1984 Oct;133(4):2025-31.
Experiments were performed to test the hypothesis that prostaglandins are crucial to the ability of an antigen-specific T cell suppressor factor to deliver a suppressive signal. In the system employed, T suppressor cells release an antigen-specific factor (TsF) that suppresses the ability of effector cells to transfer contact sensitivity (CS) skin swelling responsiveness to adoptive recipients. Culture of TsF-producing cells in the presence of indomethacin caused production of an inactive TsF that could be reconstituted by incubation of this inactive factor with low concentrations of certain prostaglandins such as PGE2 or PGE1. Subsequently, nearly all the prostaglandins were removed by dialysis, and the reconstituted TsF then acted as an antigen-specific suppressor of CS effector cells. Neither the inactive factor nor prostaglandins were suppressive alone. Furthermore, the prostaglandins are crucial to the constitution of TNBSA-F, the non-antigen-binding subunit of the TsF that probably delivers the ultimate suppressive signal. These results provide a new type of antigen-specific role for prostaglandins in immunoregulation and indicate that simple, local, hormonal molecules in physiologic concentrations can have a crucial and long-lasting role in constituting the suppressive activity of antigen-specific regulatory macromolecules released by suppressor T cells.
进行了实验以检验前列腺素对于抗原特异性T细胞抑制因子传递抑制信号的能力至关重要这一假说。在所采用的系统中，T抑制细胞释放一种抗原特异性因子（TsF），该因子可抑制效应细胞将接触敏感性（CS）皮肤肿胀反应性传递给过继受体的能力。在吲哚美辛存在的情况下培养产生TsF的细胞，会产生一种无活性的TsF，将这种无活性因子与低浓度的某些前列腺素（如PGE2或PGE1）一起孵育可使其恢复活性。随后，几乎所有的前列腺素都通过透析去除，然后恢复活性的TsF作为CS效应细胞的抗原特异性抑制剂发挥作用。无活性因子和前列腺素单独都没有抑制作用。此外，前列腺素对于TNBSA-F的构成至关重要，TNBSA-F是TsF的非抗原结合亚基，可能传递最终的抑制信号。这些结果为前列腺素在免疫调节中提供了一种新型的抗原特异性作用，并表明生理浓度的简单、局部激素分子在构成抑制性T细胞释放的抗原特异性调节大分子的抑制活性方面可发挥关键且持久的作用。
