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对乙酰氨基芴修饰的(dT-dG)n.(dC-dA)n进行的光谱研究表明其为左手构象。

Spectroscopic studies on acetylaminofluorene-modified (dT-dG)n . (dC-dA)n suggest a left-handed conformation.

作者信息

Wells R D, Miglietta J J, Kłysik J, Larson J E, Stirdivant S M, Zacharias W

出版信息

J Biol Chem. 1982 Sep 10;257(17):10166-71.

PMID:6213617
Abstract

CD spectroscopy on the double-stranded strictly alternating dinucleotide polymer (dT-dG)n . (dC-dA)n partially modified by N-acetoxy-N-acetyl-2-aminofluorene suggests a left-handed conformation in concentrated NaCl solutions. Modification of the (dT-dG)n . (dC-dA)n polymer with acetylaminofluorene is required to promote formation of the left-handed helix since high salt concentrations and several other ionic conditions, which cause a similar transition for (dG-dC)n . (dG-dC)n, are ineffective. Furthermore, substitution of dC with 5-methyl dC in (dT-dG)n . (dC-dA)n does not facilitate formation of a left-handed helix, also in contrast to results found for (dG-dC)n . (dG-dC)n. A 62-base pair tract of almost perfectly alternating (dT-dG)n . (dC-dA)n from the 3'-side of the mouse kappa immunoglobulin gene modified with acetylaminofluorene undergoes the salt-induced transition to a left-handed helix when studied within a 140-base pair restriction fragment. High NaCl concentrations alone will not cause the transition for this 62-base pair tract in this fragment nor in the recombinant plasmid pRW777, which contains this fragment.

摘要

对经N - 乙酰氧基 - N - 乙酰 - 2 - 氨基芴部分修饰的双链严格交替二核苷酸聚合物(dT - dG)n·(dC - dA)n进行圆二色光谱分析表明，在浓氯化钠溶液中其呈左手构象。用乙酰氨基芴对(dT - dG)n·(dC - dA)n聚合物进行修饰是促进左手螺旋形成所必需的，因为高盐浓度和其他几种离子条件对(dG - dC)n·(dG - dC)n会引起类似的转变，但对(dT - dG)n·(dC - dA)n却无效。此外，在(dT - dG)n·(dC - dA)n中用5 - 甲基dC取代dC也不利于左手螺旋的形成，这同样与(dG - dC)n·(dG - dC)n的结果相反。当在一个140碱基对的限制片段中进行研究时，来自小鼠κ免疫球蛋白基因3'侧的一段几乎完美交替的(dT - dG)n·(dC - dA)n的62碱基对片段，经乙酰氨基芴修饰后会发生盐诱导的向左手螺旋的转变。单独的高氯化钠浓度不会导致该片段中这62碱基对片段发生转变，在含有该片段的重组质粒pRW777中也不会发生这种转变。

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Spectroscopic studies on acetylaminofluorene-modified (dT-dG)n . (dC-dA)n suggest a left-handed conformation.对乙酰氨基芴修饰的(dT-dG)n.(dC-dA)n进行的光谱研究表明其为左手构象。
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引用本文的文献

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Mol Gen Genet. 1994 Oct 17;245(1):69-77. doi: 10.1007/BF00279752.
2
Intercalative and nonintercalative binding of large cationic porphyrin ligands to polynucleotides.大阳离子卟啉配体与多核苷酸的插入和非插入结合
Nucleic Acids Res. 1983 Sep 10;11(17):6141-54. doi: 10.1093/nar/11.17.6141.
3
Chain flexibility and hydrodynamics of the B and Z forms of poly(dG-dC).poly(dG-dC).聚（dG-dC）·聚（dG-dC）的B型和Z型的链柔性与流体动力学
Nucleic Acids Res. 1983 Mar 25;11(6):1919-30. doi: 10.1093/nar/11.6.1919.
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Stabilization of (dG-dC)n.(dG-dC)n in the Z conformation by a crosslinking reaction.通过交联反应使(dG-dC)n.(dG-dC)n稳定于Z构象。
Nucleic Acids Res. 1983 Dec 10;11(23):8421-9. doi: 10.1093/nar/11.23.8421.
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Chiral probes for the handedness of DNA helices: enantiomers of tris(4,7-diphenylphenanthroline)ruthenium(II).用于检测DNA螺旋手性的手性探针：三（4,7-二苯基菲咯啉）钌（II）的对映体
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