Pattison J R, Jackson C
J Immunol Methods. 1978;19(2-3):269-77. doi: 10.1016/0022-1759(78)90186-2.
Three new gels, Ultrogels AcA 22, AcA 34 and AcA 44 have been compared with Sephadex G-200 for the detection of rubella-specific IgM. Ultrogel AcA 44 did not achieve an adequate separation of IgM and IgG. Ultrogels AcA 22 and AcA 34 gave clear separation of IgM and IgG but only the latter could be operated at flow rates significantly higher than Sephadex G-200. With rubella convalescent sera fractionation with Ultrogel AcA 34 leads to a 2-4-fold loss of sensitivity for the detection of the first peak of rubella HI activity which is associated with recent infection. This is largely due to the occurrence of specific IgA antibody in the first peak of rubella HI activity after Sephadex G-200 fractionation but not after Ultrogel AcA 34 fractionation.
为检测风疹特异性IgM，已将三种新型凝胶Ultrogels AcA 22、AcA 34和AcA 44与Sephadex G - 200进行了比较。Ultrogel AcA 44未能充分分离IgM和IgG。Ultrogels AcA 22和AcA 34能清晰分离IgM和IgG，但只有后者能够在显著高于Sephadex G - 200的流速下运行。用风疹恢复期血清进行分馏时，使用Ultrogel AcA 34会导致检测与近期感染相关的风疹血凝抑制（HI）活性第一个峰的灵敏度损失2至4倍。这主要是因为在经Sephadex G - 200分馏后的风疹HI活性第一个峰中出现了特异性IgA抗体，而经Ultrogel AcA 34分馏后则未出现。
