编码大肠杆菌K-12外膜蛋白II的ompA基因的核苷酸序列。
Nucleotide sequence of the gene ompA coding the outer membrane protein II of Escherichia coli K-12.
作者信息
Beck E, Bremer E
出版信息
Nucleic Acids Res. 1980 Jul 11;8(13):3011-27. doi: 10.1093/nar/8.13.3011.
Abstract
A nucleotide sequence of 2271 basepairs has been determined from cloned E. coli DNA which contains ompA. Withing that sequence, starting at nucleotide 1037, an open translational reading frame encodes a protein of 367 amino acids which starting with amino acid 22 agrees with the primary structure of protein II. The preceeding 21 amino acids constitute a typical signal sequence. There is a non-translated region of 360 nucleotides in front of the translational start. The insertion point of an IS1 element 110 nucleotides upstream from the start codon and an amber codon at the position of amino acid residue 28 have been localized in the DNA from two ompA mutants.
摘要
已从含有ompA的克隆大肠杆菌DNA中确定了一段2271个碱基对的核苷酸序列。在该序列中，从核苷酸1037开始，一个开放的翻译阅读框编码一个由367个氨基酸组成的蛋白质，该蛋白质从第22个氨基酸开始与蛋白质II的一级结构一致。前面的21个氨基酸构成一个典型的信号序列。在翻译起始位点之前有一个360个核苷酸的非翻译区。在两个ompA突变体的DNA中，已定位了一个位于起始密码子上游110个核苷酸处的IS1元件的插入点以及位于氨基酸残基28位置的一个琥珀密码子。
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