Dauber J H, Rossman M D, Pietra G G, Jimenez S A, Daniele R P
Am J Pathol. 1980 Dec;101(3):595-612.
Six months after intratracheal instillation of silica, histologic, ultrastructural, cytologic, and biochemical studies were performed on the lungs of guinea pigs. The tissue response consisted of both diffuse alveolar septal infiltration with interstitial fibrosis and granulomatous infiltration with nodular fibrosis. Ultrastructural studies confirmed the presence of a mixed inflammatory exudate in the alveolar interstitium (histiocytes, neutrophils, eosinophils, and lymphocytes) and the Type II lining of cell hyperplasia. The number of lung cells recovered by lavage and the proportions of neutrophils and multinucleated cells in bronchoalveolar cells were significantly greater in experimental animals (P < .05) than in controls (intratracheal saline). Total lung collagen and collagen synthesis by cultured lung tissue were also increased in the experimental animals. Since the response of guinea pig lung to intratracheal silica included pathologic features common to human silicosis and idiopathic pulmonary fibrosis, this model has the potential for improving our understanding of both of these important clinical disorders.
在气管内注入二氧化硅六个月后，对豚鼠的肺部进行了组织学、超微结构、细胞学和生物化学研究。组织反应包括弥漫性肺泡间隔浸润伴间质纤维化以及肉芽肿浸润伴结节性纤维化。超微结构研究证实肺泡间质中存在混合性炎性渗出物（组织细胞、中性粒细胞、嗜酸性粒细胞和淋巴细胞）以及Ⅱ型肺泡上皮细胞增生。实验动物通过灌洗回收的肺细胞数量以及支气管肺泡细胞中中性粒细胞和多核细胞的比例显著高于对照组（气管内注入生理盐水）（P < 0.05）。实验动物的肺总胶原含量以及培养的肺组织中的胶原合成也有所增加。由于豚鼠肺对气管内二氧化硅的反应包括人类矽肺和特发性肺纤维化共有的病理特征，因此该模型有可能增进我们对这两种重要临床疾病的理解。
