通过细菌微小细胞原生质体融合实现酿酒酵母细胞的高效转化。
High-efficiency transformation of Saccharomyces cerevisiae cells by bacterial minicell protoplast fusion.
作者信息
Gyuris J, Duda E G
出版信息
Mol Cell Biol. 1986 Sep;6(9):3295-7. doi: 10.1128/mcb.6.9.3295-3297.1986.
Abstract
After a new transformation procedure, 10% of Saccharomyces cerevisiae cells were found to contain transforming DNA sequences. We used direct transfer of plasmid molecules by fusing bacterial minicell protoplasts to yeast protoplasts. Since the procedure significantly reduces the toxic effect of procaryotic protoplasm on the eucaryotic organism, it might be generally applicable in other systems in which transformation is inefficient or impossible.
摘要
经过一种新的转化程序后，发现10%的酿酒酵母细胞含有转化DNA序列。我们通过将细菌微小细胞原生质体与酵母原生质体融合来进行质粒分子的直接转移。由于该程序显著降低了原核原生质对真核生物的毒性作用，它可能普遍适用于其他转化效率低或无法进行转化的系统。
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本文引用的文献
1
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Nucleic Acids Res. 1982 Apr 24;10(8):2625-37. doi: 10.1093/nar/10.8.2625.
2
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J Bacteriol. 1982 Feb;149(2):542-7. doi: 10.1128/jb.149.2.542-547.1982.
3
Direct transfer of cloned genes from bacteria to mammalian cells.将克隆基因从细菌直接转移至哺乳动物细胞。
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2163-7. doi: 10.1073/pnas.77.4.2163.
4
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Methods Cell Biol. 1973;7:287-311. doi: 10.1016/s0091-679x(08)61783-5.
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Virology. 1973 Apr;52(2):456-67. doi: 10.1016/0042-6822(73)90341-3.
6
Use of minicells for bacteriophage-directed polypeptide synthesis.
Methods Enzymol. 1979;68:493-503. doi: 10.1016/0076-6879(79)68038-2.
7
Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.酵母中的转化：一种杂种克隆载体的构建及CAN1基因的分离
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Transformation of yeast by a replicating hybrid plasmid.通过复制型杂种质粒对酵母进行转化。
Nature. 1978 Sep 14;275(5676):104-9. doi: 10.1038/275104a0.
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