Wells P W, Emery D L, Hinson C A, Morrison W I, Murray M
Infect Immun. 1982 Apr;36(1):1-10. doi: 10.1128/iai.36.1.1-10.1982.
Nine different adjuvants were examined for their ability to potentiate the humoral and cell-mediated immune responses of cattle to a soluble glycoprotein antigen prepared from Trypanosoma brucei. Serological responses as measured by the Farr assay were best augmented by the oil-based adjuvants and saponin. Cell-mediated immunity, as assessed by specific lymphocyte transformation in vitro, was enhanced by all oil-based adjuvants at different intervals after immunization. Results from a challenge infection of immunized cattle with the homologous clone of T. brucei and from neutralization tests indicated that protection against infection was better correlated with specific antibodies than with cell-mediated responses. From these considerations, and the absence of tissue reactions at the site of inoculation, saponin was considered more practical than the oil-based or bacterial adjuvants for the elicitation in cattle of antibodies to purified soluble antigens.
研究了九种不同佐剂增强牛对布氏锥虫制备的可溶性糖蛋白抗原的体液免疫和细胞介导免疫反应的能力。通过Farr试验测定的血清学反应，以油基佐剂和皂苷增强效果最佳。通过体外特异性淋巴细胞转化评估的细胞介导免疫，在免疫后的不同时间间隔，所有油基佐剂均能增强。用布氏锥虫同源克隆对免疫牛进行攻击感染的结果以及中和试验表明，抗感染保护与特异性抗体的相关性优于细胞介导反应。基于这些考虑，并且接种部位没有组织反应，皂苷被认为比油基或细菌佐剂更实用，可用于在牛体内引发针对纯化可溶性抗原的抗体。
