Groffen J, Heisterkamp N, Grosveld F, Van de Ven W, Stephenson J R
Science. 1982 Jun 4;216(4550):1136-8. doi: 10.1126/science.6281890.
To define the human homolog (or homologs) of transforming sequences (v-fes gene) common to Gardner (GA) and Snyder Theilen (ST) isolates of feline sarcoma virus (FeSV), a representative library of human lung carcinoma DNA in a cosmid vector system was constructed. Three cosmid clones were isolated containing GA/ST FeSV v-fes homologous cellular sequences, within 32- to 42-kilobase cellular inserts representing 56 kilobases of contiguous human cellular DNA. Sequences both homologous to, and colinear with, GA or ST FeSV v-fes are distributed discontinuously over a region of up to 9.5 kilobases and contain a minimum of three regions of nonhomology representing probable introns. A thymidine kinase selection system was used to show that, upon transfection to RAT-2 cells, the human c-fes sequence lacked detectable transforming activity.
为了确定猫肉瘤病毒（FeSV）的加德纳（GA）分离株和斯奈德·泰伦（ST）分离株共有的转化序列（v-fes基因）的人类同源物，构建了一个在黏粒载体系统中的人肺癌DNA代表性文库。分离出三个黏粒克隆，其在代表56千碱基连续人类细胞DNA的32至42千碱基细胞插入片段中包含GA/ST FeSV v-fes同源细胞序列。与GA或ST FeSV v-fes同源且共线的序列不连续地分布在长达9.5千碱基的区域上，并包含至少三个代表可能内含子的非同源区域。使用胸苷激酶选择系统表明，在转染到RAT-2细胞后，人c-fes序列缺乏可检测到的转化活性。
