大肠杆菌中质子动力的定量测量及其与乳糖稳态积累的关系。
Quantitative measurements of the proton-motive force and its relation to steady state lactose accumulation in Escherichia coli.
作者信息
Ahmed S, Booth I R
出版信息
Biochem J. 1981 Dec 15;200(3):573-81. doi: 10.1042/bj2000573.
Abstract
The magnitude of delta psi (membrane potential), delta pH (pH gradient), lactose accumulation and cytoplasmic volume have been determined over a range of experimental conditions. A study of two probes of delta pH, benzoate and dimethyloxazolidene-2,4-dione (DMO), and four probes of delta psi, Rb+, K+, tetraphenylphosphonium (TPP+) and 3,3'-dipropylthiodicarbocyanine iodide, has been carried out. Benzoate and DMO are shown to be equivalent at pH values above the pK of DMO, but the latter may be less accurate below this pH. The cations TPP+ and Rb+ were found, by a number of criteria, to be equivalent, and TPP+ may be used in cells not pretreated with EDTA. These studies are an essential prerequisite to the use of TPP+ as a quantitative probe in untreated cells.
摘要
在一系列实验条件下，已测定了Δψ（膜电位）、ΔpH（pH梯度）、乳糖积累和细胞质体积的大小。对两种ΔpH探针（苯甲酸盐和二甲基恶唑烷 - 2,4 - 二酮（DMO））以及四种Δψ探针（Rb⁺、K⁺、四苯基鏻（TPP⁺）和3,3'-二丙基硫代二羰花青碘化物）进行了研究。结果表明，在pH值高于DMO的pK时，苯甲酸盐和DMO等效，但在该pH值以下，后者可能不太准确。通过多种标准发现阳离子TPP⁺和Rb⁺等效，并且TPP⁺可用于未用EDTA预处理的细胞。这些研究是将TPP⁺用作未处理细胞中定量探针的必要前提。
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