从产抗生素链霉菌中克隆的抗性决定因子的生化特性分析。
Biochemical characterization of resistance determinants cloned from antibiotic-producing streptomycetes.
作者信息
Thompson C J, Skinner R H, Thompson J, Ward J M, Hopwood D A, Cundliffe E
出版信息
J Bacteriol. 1982 Aug;151(2):678-85. doi: 10.1128/jb.151.2.678-685.1982.
Abstract
Determinants of antibiotic resistance have been cloned from four antibiotic-producing streptomycetes into Streptomyces lividans. Biochemical analyses of resistant clones revealed the presence of enzymes that had previously been characterized as likely resistance determinants in the producing organisms. These included: 23S rRNA methylases from S. azureus and S. erythreus, which confer resistance to thiostrepton and erythromycin, respectively; viomycin phosphotransferase from S. vinaceus; and aminoglycoside phosphotransferase and acetyltransferase from the neomycin producer S. fradiae. In general, the levels of antibiotic resistance of the clones were similar to those of the producing organisms. Although the two aminoglycoside-modifying enzymes from S. fradiae could independently confer only low-level resistance to neomycin, the presence of both enzymes in the same strain resulted in a level of resistance comparable with that of the producing organism.
摘要
已从四种产抗生素的链霉菌中克隆出抗生素抗性决定簇，并导入变铅青链霉菌中。对抗性克隆进行的生化分析表明，存在一些酶，这些酶在产生抗生素的生物体中先前已被鉴定为可能的抗性决定因素。其中包括：天蓝链霉菌和红霉素链霉菌的23S rRNA甲基化酶，分别赋予对硫链丝菌素和红霉素的抗性；酒红色链霉菌的紫霉素磷酸转移酶；以及新霉素产生菌弗氏链霉菌的氨基糖苷磷酸转移酶和乙酰转移酶。一般来说，克隆的抗生素抗性水平与产生抗生素的生物体相似。虽然来自弗氏链霉菌的两种氨基糖苷修饰酶单独只能赋予对新霉素的低水平抗性，但同一菌株中同时存在这两种酶会导致抗性水平与产生抗生素的生物体相当。
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