利用酶标记抗原检测抗爱泼斯坦-巴尔病毒免疫球蛋白M抗体。
Detection of immunoglobulin M antibody to Epstein-Barr virus by use of an enzyme-labeled antigen.
作者信息
Schmitz H
出版信息
J Clin Microbiol. 1982 Aug;16(2):361-6. doi: 10.1128/jcm.16.2.361-366.1982.
Abstract
Immunoglobulin M (IgM) antibodies to Epstein-Barr virus were detected by using microtiter plates coated with anti-mu-chain antiserum and enzyme-labeled Epstein-Barr virus antigen. Optimum conditions for labeling were determined. The addition of unlabeled control antigen to the enzyme-labeled antigen was effective in reducing background reactions. Rheumatoid factor no longer interfered. Blocking of specific IgM antibody by IgG also was no longer observed. Four different methods for the detection of acute Epstein-Barr virus infections were compared. A combination of the enzyme-labeled antigen-IgM test with the detection of antibodies to either Epstein-Barr nuclear antigen or heterophile antigen was highly sensitive and specific. By changing the solid phase, IgA antibodies to Epstein-Barr virus could be detected.
摘要
采用包被抗μ链抗血清的微量滴定板和酶标记的爱泼斯坦-巴尔病毒抗原检测抗爱泼斯坦-巴尔病毒的免疫球蛋白M（IgM）抗体。确定了标记的最佳条件。向酶标记抗原中加入未标记的对照抗原可有效减少背景反应。类风湿因子不再产生干扰。也不再观察到IgG对特异性IgM抗体的阻断作用。比较了四种检测急性爱泼斯坦-巴尔病毒感染的不同方法。酶标记抗原-IgM试验与检测爱泼斯坦-巴尔核抗原或嗜异性抗原的抗体相结合，具有高度的敏感性和特异性。通过改变固相，可以检测到抗爱泼斯坦-巴尔病毒的IgA抗体。
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