Ito Y, Tokuda H, Morigaki T, Shimizu K, Kawana T, Sanada S, Yoshida O
Cancer Lett. 1984 Jun;23(2):129-34. doi: 10.1016/0304-3835(84)90145-9.
During the screening of natural and physiological products for their Epstein-Barr virus (EBV)-activating potency, we found that a considerable number of human semen specimens obtained from infertility clinics (30 cases) possess a marked capacity to induce EBV early antigen (EA) in non-producer Raji cell system when assayed in combination with n-butyrate. The EBV EA-inducing activity of the semen samples was comparable to that of the most efficient EBV EA inducers, the plant diterpene esters such as 12-O-tetradecanoylphorbol-13-acetate (TPA). Since many, if not all, of such active agents show overlapping with the tumor promoters in their biological reactions, these findings may provide a new insight for assessing the role of semen in the etiology of certain types of human genital malignancies.
在筛选天然和生理产物的爱泼斯坦-巴尔病毒（EBV）激活能力时，我们发现，从不孕不育诊所获取的大量人类精液标本（30例），在与正丁酸盐联合检测时，在非产生型Raji细胞系统中具有显著的诱导EBV早期抗原（EA）的能力。精液样本的EBV EA诱导活性与最有效的EBV EA诱导剂，如植物二萜酯12-O-十四酰佛波醇-13-乙酸酯（TPA）相当。由于许多（如果不是全部）此类活性剂在其生物学反应中与肿瘤启动子存在重叠，这些发现可能为评估精液在某些类型人类生殖器恶性肿瘤病因学中的作用提供新的见解。
