从医院饮用水样本中分离嗜肺军团菌：直接平板接种法与豚鼠接种法的比较
Isolation of Legionella pneumophila from hospital potable water specimens: comparison of direct plating with guinea pig inoculation.
作者信息
Edelstein P H, Snitzer J B, Finegold S M
出版信息
J Clin Microbiol. 1982 Jun;15(6):1092-6. doi: 10.1128/jcm.15.6.1092-1096.1982.
Abstract
Eighty-five potable water samples were analyzed for the presence of Legionella pneumophila by direct plating and by guinea pig inoculation methods. Direct plating methods utilized antimicrobial-containing media with and without acid treatment of specimens before inoculation. Thirty-three specimens were culture positive for L. pneumophila by one or both techniques. A total of 14 specimens were positive for L. pneumophila by direct plating only, and 2 specimens were positive for L. pneumophila by guinea pig inoculation only. The sensitivity of direct plating is significantly greater than that of guinea pig inoculation when isolating L. pneumophila from potable water (P less than 0.01).
摘要
采用直接平板接种法和豚鼠接种法对85份饮用水样本进行嗜肺军团菌检测。直接平板接种法使用了含抗菌剂的培养基，接种前样本分别经过和未经过酸处理。两种方法中，有33份样本检测出嗜肺军团菌呈阳性。仅直接平板接种法检测出嗜肺军团菌呈阳性的样本有14份，仅豚鼠接种法检测出嗜肺军团菌呈阳性的样本有2份。从饮用水中分离嗜肺军团菌时，直接平板接种法的灵敏度显著高于豚鼠接种法（P<0.01）。
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