Woolfrey B F, Lally R T, Ederer M N, Quall C O
J Clin Microbiol. 1984 Dec;20(6):1053-9. doi: 10.1128/jcm.20.6.1053-1059.1984.
The AutoMicrobic system (AMS) (Vitek Systems, Inc., Hazelwood, Mo.) was compared with the API-20E system for the identification of gram-negative bacilli by using 380 stock clinical isolates and 377 immediately encountered fresh clinical isolates. For the stock isolates, with Enterobacteriaceae-Plus Biochemical Cards and automated interpretation, 364 (95.8%) were in agreement to the species level. For the fresh clinical isolates, agreement at the genus and species levels was 89.7 and 85.9%, respectively, when Enterobacteriaceae-Plus Cards were interpreted by the AMS. Manual interpretation of Enterobacteriaceae-Plus Biochemical Cards improved species level agreement to 91.0%. Subsequent retesting of all discrepant isolates with the Gram-Negative Identification Card resulted in significant improvement of results, and for the stock and fresh clinical isolates, species level agreement was 98.7 and 97.3%, respectively. AMS susceptibility testing was evaluated by comparing ampicillin and cephalothin MICs determined in parallel by AMS and a reference broth microdilution test for stock isolates, and by comparison of AMS and standardized disk agar diffusion test results for fresh clinical isolates. For the stock isolates, AMS mean integer MICs approximated microdilution mean integer MICs with AMS, providing excellent MIC replicability. For ampicillin and cephalothin, 50 and 46.8%, respectively, of AMS integer MICs were within +/- 1 microgram/ml of the reference values, and 89.3 and 63.1% of AMS integer MICs were within +/- 2 micrograms/ml of the reference values. For the fresh clinical isolates, AMS and reference results were in disagreement for 4.5% of the antimicrobial agents tested, with 2.3% as a combination of "major" and "very major" errors.
使用380株临床保存菌株和377株刚获得的新鲜临床分离株，将自动微生物系统（AMS）（Vitek系统公司，密苏里州黑兹尔伍德）与API-20E系统用于革兰氏阴性杆菌的鉴定进行比较。对于保存菌株，使用肠杆菌科+生化卡并进行自动判读时，364株（95.8%）在种水平上一致。对于新鲜临床分离株，当AMS对肠杆菌科+卡进行判读时，属和种水平的一致率分别为89.7%和85.9%。对肠杆菌科+生化卡进行人工判读可将种水平的一致率提高到91.0%。随后用革兰氏阴性鉴定卡对所有有差异的分离株进行重新检测，结果有显著改善，对于保存菌株和新鲜临床分离株，种水平的一致率分别为98.7%和97.3%。通过比较AMS和参考肉汤微量稀释法平行测定的保存菌株的氨苄西林和头孢噻吩MIC，以及比较AMS和新鲜临床分离株的标准化纸片琼脂扩散试验结果，对AMS药敏试验进行评估。对于保存菌株，AMS平均整数MIC与微量稀释法的AMS平均整数MIC相近，具有出色的MIC重复性。对于氨苄西林和头孢噻吩，分别有50%和46.8%的AMS整数MIC在参考值的±1微克/毫升范围内，89.3%和63.1%的AMS整数MIC在参考值的±2微克/毫升范围内。对于新鲜临床分离株，在4.5%的测试抗菌药物中，AMS和参考结果不一致，其中2.3%为“主要”和“非常主要”错误的组合。
