Anthony R S, Farquharson M, MacSween R N
J Clin Pathol. 1983 Nov;36(11):1302-8. doi: 10.1136/jcp.36.11.1302.
Using an indirect immunofluorescence technique, circulating liver membrane antibodies against normal rabbit hepatocytes and ethanol-altered rabbit hepatocytes have been sought in a series of patients with histologically confirmed alcoholic liver disease. Liver membrane antibodies against normal hepatocytes were found in 18 (28%) of the 65 sera examined, but with ethanol-altered hepatocytes as substrate liver membrane antibodies were found in 48 (74%) of the sera. Isolation of F(ab')2 fragments confirmed that the positive results were due to antibody binding. Liver membrane antibodies against ethanol-altered hepatocytes are peculiar to alcoholic liver disease, and there is a similar incidence in the various histological types of alcoholic liver disease. Absorption studies suggest that the liver membrane antibodies are directed against new or altered antigens which are not present in normal hepatocytes. These new or altered antigens may also appear after pretreatment with other primary alcohols and seem likely to be induced by a haptenic effect of the alcohol or a metabolic break-down product. These studies represent a novel approach to the further investigation of the possible role of immunological mechanisms in alcohol-induced liver injury.
采用间接免疫荧光技术，在一系列经组织学确诊的酒精性肝病患者中，寻找针对正常兔肝细胞和乙醇处理过的兔肝细胞的循环肝细胞膜抗体。在所检测的65份血清中，18份（28%）发现了针对正常肝细胞的肝细胞膜抗体，但以乙醇处理过的肝细胞为底物时，48份（74%）血清中发现了肝细胞膜抗体。F(ab')2片段的分离证实阳性结果是由于抗体结合所致。针对乙醇处理过的肝细胞的肝细胞膜抗体是酒精性肝病所特有的，在各种组织学类型的酒精性肝病中发生率相似。吸收研究表明，肝细胞膜抗体针对的是正常肝细胞中不存在的新抗原或改变后的抗原。这些新抗原或改变后的抗原在用其他伯醇预处理后也可能出现，似乎很可能是由酒精的半抗原效应或代谢分解产物诱导产生的。这些研究代表了一种新方法，可进一步研究免疫机制在酒精性肝损伤中可能发挥的作用。
