酒精性肝病患者体内针对经酒精处理的兔肝细胞的血清抗体。
Serum antibodies against alcohol-treated rabbit hepatocytes in patients with alcoholic liver disease.
作者信息
Izumi N, Sato C, Hasumura Y, Takeuchi J
出版信息
Clin Exp Immunol. 1985 Sep;61(3):585-92.
The existence of antibodies against alcohol-treated rabbit hepatocytes in sera of patients with alcoholic liver disease was investigated utilizing a 125I-labelled protein A assay. The sera, after two absorptions with normal rabbit hepatocytes, were incubated with hepatocytes isolated from rabbits which had been treated for 4 days with a daily dose of ethanol (1 g/kg body weight) intravenously. Serum antibodies against alcohol-treated hepatocytes were detected in 21 of 55 patients with alcoholic liver disease; this was associated with a distinct, mixed granular and linear fluorescence staining the surface of alcohol-treated hepatocytes. By contrast, none of 23 patients with other liver diseases who had no history of excessive alcohol intake showed the antibodies in their sera. The values of the antibodies of patients with alcoholic active cirrhosis and alcoholic hepatitis were significantly higher than those of other types of alcoholic liver disease. These results showed that antibodies against alcohol-treated hepatocytes are present in alcoholic liver disease with inflammation. The role of these antibodies on liver cell damage in alcoholic liver disease remains to be clarified.
利用125I标记的蛋白A分析法，研究了酒精性肝病患者血清中抗酒精处理兔肝细胞抗体的存在情况。血清先用正常兔肝细胞进行两次吸收，然后与从每天静脉注射乙醇（1克/千克体重）处理4天的兔子分离出的肝细胞一起孵育。55例酒精性肝病患者中有21例检测到抗酒精处理肝细胞的血清抗体；这与酒精处理肝细胞表面明显的、混合的颗粒状和线性荧光染色有关。相比之下，23例无过量饮酒史的其他肝病患者血清中均未显示出这些抗体。酒精性活动性肝硬化和酒精性肝炎患者的抗体值明显高于其他类型的酒精性肝病患者。这些结果表明，在有炎症的酒精性肝病中存在抗酒精处理肝细胞的抗体。这些抗体在酒精性肝病肝细胞损伤中的作用仍有待阐明。
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