Neuberger J, Crossley I R, Saunders J B, Davis M, Portmann B, Eddleston A L, Williams R
Gut. 1984 Mar;25(3):300-4. doi: 10.1136/gut.25.3.300.
Circulating antibodies reacting specifically with hepatocytes isolated from ethanol pretreated rabbits have been demonstrated by two techniques - induced cytotoxicity and immunofluorescence. In the cytotoxicity assay antibodies were found in seven of 19 (39%) of patients with alcoholic fatty liver (with or without fibrosis), six of 13 (46%) of those with alcoholic hepatitis, 15 of 36 (43%) of those with cirrhosis, and seven of 14 patients (50%) of those with hepatitis and cirrhosis. In the immunofluorescence studies, nine of 15 sera induced a granular pattern of fluorescence on the ethanol pretreated hepatocytes; two sera which induced significant cytotoxicity did not induce immunofluorescence. No ethanol related antibodies were found in normal individuals or in patients with other types of acute or chronic liver disease. These results show that antibodies directed against ethanol altered liver cell determinants are present in the serum of 43% of patients with alcoholic liver disease, and suggest a mechanism whereby chronic alcohol consumption may, by inducing antigenic changes in hepatocyte membranes, trigger a cell damaging immune reaction.
通过诱导细胞毒性和免疫荧光这两种技术，已证实从乙醇预处理的兔子中分离出的肝细胞存在特异性循环抗体。在细胞毒性试验中，19例酒精性脂肪肝（伴有或不伴有纤维化）患者中有7例（39%）、13例酒精性肝炎患者中有6例（46%）、36例肝硬化患者中有15例（43%）以及14例肝炎和肝硬化患者中有7例（50%）检测到抗体。在免疫荧光研究中，15份血清中有9份在乙醇预处理的肝细胞上诱导出颗粒状荧光模式；两份诱导出显著细胞毒性的血清未诱导出免疫荧光。在正常个体或其他类型急性或慢性肝病患者中未发现与乙醇相关的抗体。这些结果表明，43%的酒精性肝病患者血清中存在针对乙醇改变的肝细胞决定簇的抗体，并提示一种机制，即长期饮酒可能通过诱导肝细胞膜的抗原变化，引发细胞损伤性免疫反应。
