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在一项前瞻性临床试验中,杜邦裂解离心系统的储存对细菌和真菌回收率的影响。

Effect of storage of the du Pont lysis-centrifugation system on recovery of bacteria and fungi in a prospective clinical trial.

作者信息

Stockman L, Roberts G D, Ilstrup D M

出版信息

J Clin Microbiol. 1984 Feb;19(2):283-5. doi: 10.1128/jcm.19.2.283-285.1984.

Abstract

A commercially available lysis-centrifugation system was compared with a conventional biphasic brain heart infusion medium in a prospective clinical study of 5,125 fungal blood cultures. Recovery rates were compared between two time periods to assess the effect of 25 degrees C storage before processing by the lysis-centrifugation system. The lysis-centrifugation tubes processed within 9 h showed a significantly higher yield (3.4 versus 1.49%) for yeasts (Candida glabrata), filamentous fungi (Histoplasma capsulatum), and bacteria (8.84 versus 7.34%) (Klebsiella pneumoniae and Serratia marcescens) than did those processed after 9 h.

摘要

在一项对5125份真菌血培养样本的前瞻性临床研究中,将一种市售的裂解离心系统与传统的双相脑心浸液培养基进行了比较。比较了两个时间段的回收率,以评估在通过裂解离心系统处理之前25摄氏度保存的影响。在9小时内处理的裂解离心管对酵母(光滑念珠菌)、丝状真菌(荚膜组织胞浆菌)和细菌(肺炎克雷伯菌和粘质沙雷氏菌)的回收率显著更高(分别为3.4%对1.49%、8.84%对7.34%),而9小时后处理的回收率则较低。

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