通过高压液相色谱法测定反应产物来鉴定氨基糖苷乙酰化酶。
Identification of aminoglycoside-acetylating enzymes by high-pressure liquid chromatographic determination of their reaction products.
作者信息
Lovering A M, White L O, Reeves D S
出版信息
Antimicrob Agents Chemother. 1984 Jul;26(1):10-2. doi: 10.1128/AAC.26.1.10.
Abstract
A method to identify the aminoglycoside-acetyltransferase (AAC) enzymes AAC(3), AAC(2') and AAC(6') by high-pressure liquid chromatographic characterization of their products of reaction with tobramycin or sisomicin is described. Conditions are given for the chromatography of kanamycin A, netilmicin, neomycin, and apramycin, and their products of reaction, if any, with the three AAC enzymes are listed.
摘要
描述了一种通过对与妥布霉素或西索米星反应产物进行高压液相色谱表征来鉴定氨基糖苷乙酰转移酶（AAC）酶AAC(3)、AAC(2')和AAC(6')的方法。给出了卡那霉素A、奈替米星、新霉素和阿普拉霉素的色谱条件，并列出了它们与三种AAC酶反应的产物（如有）。
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本文引用的文献
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R-factor mediated gentamicin resistance: A new enzyme which modifies aminoglycoside antibiotics.R 因子介导的庆大霉素耐药性：一种修饰氨基糖苷类抗生素的新酶。
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Gentamicin transposons.庆大霉素转座子
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HPLC in clinical microbiology laboratories.临床微生物学实验室中的高效液相色谱法。
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Outbreaks of hospital infection in southwest England caused by gentamicin-resistant Serratia marcescens.英格兰西南部由耐庆大霉素的粘质沙雷氏菌引起的医院感染暴发。
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5
Aminoglycoside resistance in bacteria medicated by gentamicin acetyltransferase II, an enzyme modifying the 2'-amino group of aminoglycoside antibiotics.由庆大霉素乙酰转移酶II介导的细菌对氨基糖苷类抗生素的耐药性，该酶可修饰氨基糖苷类抗生素的2'-氨基基团。
Biochemistry. 1974 Jan 29;13(3):598-603. doi: 10.1021/bi00700a030.
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Purification and properties of gentamicin acetyltransferase I.庆大霉素乙酰转移酶I的纯化及性质
Biochemistry. 1976 Jan 13;15(1):125-31. doi: 10.1021/bi00646a019.
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