对烯丙醇具有抗性的中国仓鼠体细胞的乙醇脱氢酶突变体
Alcohol dehydrogenase mutants of Chinese hamster somatic cells resistant to allyl alcohol.
作者信息
Thirion J P, Talbot B
出版信息
Genetics. 1978 Feb;88(2):343-56. doi: 10.1093/genetics/88.2.343.
Abstract
Alcohol dehydrogenase (alcohol: NAD oxidoreductase, E.C. 1.1.1.1.) mutants of Chinese hamster somatic cells were isolated as resistant to allyl alcohol (ALLR). The ALLR phenotypes of the mutant clones were reproducible with high fidelity and stable over long intervals of growth in the absence of the selecting drug. Several mutants, Adh-1, Adh-2, Adh-9 and Adh-13, resistant to allyl alcohol were characterized. They have between 15 and 40% of the alcohol dehydrogenase activity of the wild-type cell lines. This phenotype is therefore a useful marker to analyze gene segregation of somatic cell mutations and to study the expression of the genes involved in the metabolism of ethanol in mammalian cells.
摘要
通过分离对烯丙醇具有抗性的（ALLR）中国仓鼠体细胞酒精脱氢酶（酒精：NAD氧化还原酶，E.C. 1.1.1.1.）突变体。突变克隆的ALLR表型具有高保真度且在无选择药物的长时间生长过程中稳定。对几种对烯丙醇具有抗性的突变体Adh-1、Adh-2、Adh-9和Adh-13进行了表征。它们具有野生型细胞系酒精脱氢酶活性的15%至40%。因此，这种表型是分析体细胞突变基因分离以及研究哺乳动物细胞中乙醇代谢相关基因表达的有用标记。
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