Barker P E, Ruddle F H, Royer H D, Acuto O, Reinherz E L
Science. 1984 Oct 19;226(4672):348-9. doi: 10.1126/science.6435246.
A complementary DNA probe corresponding to the beta-chain gene of Ti, the human T lymphocyte receptor, has been molecularly cloned. The chromosomal origin of the Ti beta gene was determined with the complementary DNA by screening a series of 12 cell hybrid (mouse X human) DNA's containing overlapping subsets of human chromosomes. DNA hybridization (Southern) experiments showed that the human Ti beta gene resides on chromosome 7 and is thus not linked to the immunoglobulin loci or to the major histocompatibility locus in humans.
一种与人类T淋巴细胞受体Ti的β链基因相对应的互补DNA探针已被分子克隆。通过筛选一系列包含人类染色体重叠子集的12种细胞杂交（小鼠×人类）DNA，用该互补DNA确定了Tiβ基因的染色体起源。DNA杂交（Southern）实验表明，人类Tiβ基因位于7号染色体上，因此与人类的免疫球蛋白基因座或主要组织相容性基因座没有连锁关系。
