Fuke M, Dennis K J, Busch H
Mol Gen Genet. 1981;182(1):25-30. doi: 10.1007/BF00422762.
Two Charon 4A lambda bacteriophage clones were characterized which contain all and part o the 18S ribosomal DNA of the rat. One clone contained two Eco RI fragments which include the whole 18S ribosomal RNA region and part of 28S ribosomal RNA region. The other clone contained an Eco RI fragment which covers part of 18S ribosomal RNA region. There were differences between the two clones in the non-transcribed spacer regions suggesting that there is heterogeneity in the non-transcribed spacer regions of rat ribosomal genes. The restriction maps of the two clones were compared to the restriction map of the cloned mouse ribosomal DNA. Eco RI, Hind III, Pst I, and Bam HI sites in 18S ribosomal RNA regions were in the same places in mouse and rat DNA but the restriction sites in the 5'-spacer regions were different.
对两个卡隆4A λ噬菌体克隆进行了表征，它们包含大鼠18S核糖体DNA的全部和部分序列。一个克隆包含两个Eco RI片段，其中包括整个18S核糖体RNA区域和部分28S核糖体RNA区域。另一个克隆包含一个Eco RI片段，该片段覆盖18S核糖体RNA区域的一部分。两个克隆在非转录间隔区存在差异，这表明大鼠核糖体基因的非转录间隔区存在异质性。将这两个克隆的限制性图谱与克隆的小鼠核糖体DNA的限制性图谱进行了比较。18S核糖体RNA区域中的Eco RI、Hind III、Pst I和Bam HI位点在小鼠和大鼠DNA中的位置相同，但5'-间隔区的限制性位点不同。
