用重组人α干扰素处理的小鼠细胞中沙眼衣原体生长周期的超微结构分析

Ultrastructural analysis of the growth cycle of Chlamydia trachomatis in mouse cells treated with recombinant human alpha-interferons.

作者信息

de la Maza L M, Goebel J M, Czarniecki C W, Peterson E M

出版信息

Exp Mol Pathol. 1984 Oct;41(2):227-35. doi: 10.1016/0014-4800(84)90039-x.

Abstract

The effect of two recombinant human hybrid interferons (IFNs), IFN-alpha AD (BglII) and IFN-alpha DA (BglII), on the growth cycle of Chlamydia trachomatis in a murine (McCoy) cell line was investigated. Ultrastructural analysis indicated that IFN-alpha AD inhibited the growth of chlamydia while IFN-alpha DA-treated cells did not significantly differ from the control monolayers. Treatment of the chlamydia-infected monolayers with IFN-alpha AD resulted in an inhibition in the transformation of elementary bodies to reticulate bodies with a consequent marked decrease in the number of chlamydia inclusions. Furthermore, chlamydial inclusions in the IFN-alpha AD-treated cells contained fewer and more immature chlamydial forms than the control or the IFN-alpha DA-treated cells. Secondary infection occurred in the IFN-alpha DA and in the control monolayer, but no such phenomena was detected in the IFN-alpha AD-treated McCoy cells indicating a loss of infectivity of the chlamydial organisms. From this study it can be concluded that purified recombinant human hybrid IFNs may exert an inhibitory effect on the growth cycle of C. trachomatis in a mouse cell line. This inhibition occurs primarily at the point of transformation from elementary to reticulate body.

摘要

研究了两种重组人杂交干扰素（IFN），即IFN-α AD（BglII）和IFN-α DA（BglII）对沙眼衣原体在鼠（McCoy）细胞系中生长周期的影响。超微结构分析表明，IFN-α AD抑制衣原体生长，而经IFN-α DA处理的细胞与对照单层细胞无显著差异。用IFN-α AD处理衣原体感染的单层细胞，可抑制原体向网状体的转化，从而使衣原体包涵体数量显著减少。此外，与对照或经IFN-α DA处理的细胞相比，经IFN-α AD处理的细胞中的衣原体包涵体所含的衣原体形式更少且更不成熟。IFN-α DA处理组和对照组单层细胞发生了二次感染，但在经IFN-α AD处理的McCoy细胞中未检测到此类现象，这表明衣原体生物体的感染性丧失。从这项研究可以得出结论，纯化的重组人杂交干扰素可能对沙眼衣原体在小鼠细胞系中的生长周期产生抑制作用。这种抑制主要发生在从原体向网状体转化的阶段。